- May 25, 2012 at 3:17 am #83016
ThomasMemberTopics: 71Replies: 605
I just found this page and read about this DNA test.
What do you think about it. Could this be something for us to get
a clear diagnosis? I feel it is very important to have a diagnosis and not guessing around before entering the battle. You have to know whom you are fighting against.
ThomasMay 25, 2012 at 7:22 am #83030
JavizyMemberTopics: 20Replies: 945
Try reading this review: Techniques used to characterize the gut microbiota: a guide for the clinician.
Although PCR has been a huge technical advance across the medical field, it has limitations; each physical, chemical, and biological step—from retrieving a sample to the resulting 16S rRNA amplicons—represents a potential source of bias (Figure 3).34 For example, differential lysis of microbial cells can affect the final apparent microbiota composition; Gram-positive organisms typically require rigorous conditions to lyse the bacterial cell wall (which is thicker than in Gram-negative bacteria), while these same conditions may cause excessive fragmentation of Gram-negative chromosomal DNA.33 Another major limitation of PCR is that primers must be designed to target all phyla.
PCR alone is unreliable for quantification of the DNA present in a sample; quantitative PCR (qPCR), also known as real-time PCR, not only amplifies DNA but also reliably quantifies the amount of DNA present. The technique used is similar to that described above for standard PCR but the reaction mixture contains a compound that fluoresces when it binds to double-stranded DNA, that is, the PCR product.
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